We developed a series of fluorescent probes, named fluorescent D-amino acids (FDAAs), for bacterial peptidoglycan labeling. These probes incorporate to peptidoglycan through the activity of cell wall synthases, indicating the formation and remodeling of peptidoglycan in situ.
Figure 1 legends: FDAAs labeling in Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative). Hsu et, al. 10.1039/C7SC01800B
Figure 2 legends: Successive FDAA labeling in polar growing bacteria, Streptomyces venezuelae. This labeling method is also known as virtual time-lapse labeling. Hsu et, al. 10.1039/C7SC01800B
Figure 3 legends: Structures and spectra of FDAAs developed in the VanNieuwenhze Lab.
Figure 4 legends: Photochemical and physical properties of FDAAs. Hsu et, al. 10.1039/C7SC01800B
FDAA application and reference
Table 1. Examples of FDAAs applications in recent studies. Sub-headings classify the use of FDAAs in the experiment designs of each study.